Here's How To Actually Clean Your Disgusting Phone. Caroline Kee / Ashley McGetrick / Via BuzzFeed. The alcohol in the solution will allow it to dry pretty quickly, so you'll be ready to. Biacore systems exploit the phenomenon of surface plasmon resonance (SPR) to monitor the. Curve indicate the solutions that pass over the sensor surface. Run Desorb followed by Sanitize according to the instrument maintenance.
Flow change
Change in flow rate can cause a drift in the sensorgram, which will level out over time (5 - 30 minutes). The duration of this effect depends on the type of sensor chip and ligand bound to it. The effect can also be seen at the start of a sensorgram. One remedy is to start the sensorgram at the desired flow rate and add a WAIT command of 15 minutes to minimize the effect.
Change in flow rate can cause a drift in the sensorgram, which will level out over time (5 - 30 minutes). The duration of this effect depends on the type of sensor chip and ligand bound to it. The effect can also be seen at the start of a sensorgram. One remedy is to start the sensorgram at the desired flow rate and add a WAIT command of 15 minutes to minimize the effect.
Drift and shift
Drift and shift are often caused by differences in flow buffer. Make sure that one batch of buffer is used and the PRIME-command is performed after buffer change. Another cause can be the build-up of small air bubbles in the flow channels. Especially at low flow rates (< 10 µl/min) the small air bubbles are not driven out fast enough, giving them time to grow until they come visible in the sensorgram. At high temperatures, e.g. 37°C air bubbles are easily formed. Thoroughly degassed buffers will help to minimize the effect. When using a method, the UNCLOG command can be used to flush out small air bubbles between cycles. In addition, sufficient equilibration after immobi¬li¬zation is necessary because of the chemicals used. It is recommended to equilibrate the system over night after immobilization.
Drift and shift are often caused by differences in flow buffer. Make sure that one batch of buffer is used and the PRIME-command is performed after buffer change. Another cause can be the build-up of small air bubbles in the flow channels. Especially at low flow rates (< 10 µl/min) the small air bubbles are not driven out fast enough, giving them time to grow until they come visible in the sensorgram. At high temperatures, e.g. 37°C air bubbles are easily formed. Thoroughly degassed buffers will help to minimize the effect. When using a method, the UNCLOG command can be used to flush out small air bubbles between cycles. In addition, sufficient equilibration after immobi¬li¬zation is necessary because of the chemicals used. It is recommended to equilibrate the system over night after immobilization.